時間:2005-05-10
瀏覽量:4013
余文祥 李菊升 李崇珍 余 蕾 黃云芝 云南省瘧疾防治研究所(思茅665000)
全文發(fā)表于《中國寄生蟲防治雜志》1993年第6卷第3期161-162頁
提要:用間日瘧病人血,離體感染中華按蚊,分離子孢子,用10~12萬條子孢子/次的量,免疫BALB/c小鼠,抗體效價均達到1:640,取其脾細胞作融合試驗,已培養(yǎng)出抗體滴度高、特異性強的抗間日瘧原蟲子孢子單克隆抗體(McAb)。
關鍵詞:間日瘧原蟲子孢子,免疫
STUDIES ON IMMUNIZATION OF BALB/c MICE WITH PLASMODIUM VIVAX SPOROZOITES
Yu Wen-Xiang, Li Ju-Sheng, Li Chong-Zhen, Yu Lei, Huang Yun-Zhi
ABSTRACT. The blood taken from Plasmodium vivax patients was used for memebrane-feeding An. Sinensis wiedmanns. 15 day later or so, the sporozites were separated. Each mouse was immunized with100-200 thousand sporozoites per time according to a certain schedual. All the titers of antibody to sporozoites from immunized mice reached to 1:640. The spleen cells from immunized mice were fused with SP2/0 cells to obtain the monoclonal antibodies to P. vivax sporozoites with high titers and specificity.
Key words: P.vivax sporozoite,immunization,mouse,monoclonal antibody
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