惡性瘧原蟲配子體體外培養(yǎng)實驗報告
時間：2005-05-11
瀏覽量：4262
朱兆鴻 黃開國 高白荷 李宗惠
云南省瘧疾防治研究所 思茅 665000
全文發(fā)表于《實用寄生蟲病雜志》1994年2卷2期30-31頁
提要：本文報告用蠟燭缸平皿法培養(yǎng)惡性原蟲配子體，對云南分離的FCCBH1／YN和FccMD1兩株進行培養(yǎng)獲得成功，兩株分別在4d和1d出現(xiàn)配子體，16d和10d達高峰。最高密度分別為135和123/10000RBC，V期配子體最高密度為20和30/10000RBC，平均8.3和4.3/10000RBC。雌雄配子體比例為9:1和5:1，雄配子體可見出絲1－6條，表明配子體已達到成熟階段，為國內首次報道。
關鍵詞：惡性瘧原蟲，配子體，體外培養(yǎng)
GAMETOGENESIS IN CONTINUOUS CULTURE OF PLASMODIUM FALCIPARUM
Zhu Zhaohong, Huang Kaiguo, Gao Baihe, Li Zonghui
ABSTRACT：Continuous culture of two isolates of Plasmodium falciparum collected from two malaria patients in Yunnan was carried out with the established candle jar method. Young gametocyte first appeared on day 4 or 1 and the gametocytes peaked on day 16 or 10.The peak gametocytes parasitemina reached 135 and 123/10000 RBC respectively for two isolates. The highest density of matured gametocytes was 20 and 13/10000 RBC and the ratio between macrogamete and microgamete was9:1－5:1. Rounded microgametes with 1-6 flagella were clearly seen indicating gametocytes attained to sexual maturity. But experimental infection of An.dirus and An.minimus with the viable cultured parasites was unsuccessful.
Key words：Plasmodium falciparum,gametocyte, in vitro cultivation
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